Antibodies for immunohistochemistry

Immunohistochemistry is a procedure aimed at detecting a specific antigen in a tissue. This technique allows for the visualization and identification of the location of specific proteins in histological preparations under the microscope.

Antigen detection can be direct or indirect. In the direct method, the antibody added to the sample may have been previously conjugated with substances that enable its detection under the microscope, such as fluorescent substances or enzymes that can catalyze a reaction, leaving a visible product where the antibody binds. In the indirect method, secondary antibodies conjugated with these substances are used to detect and bind to the primary antibody.

Immunohistochemistry is not only used in basic research but has also revolutionized disease diagnosis. This technique is especially useful in cancer, where it is used not only for general diagnosis but also to distinguish between tumor subtypes.